期刊
INSECT MOLECULAR BIOLOGY
卷 23, 期 1, 页码 78-97出版社
WILEY
DOI: 10.1111/imb.12064
关键词
Lygus lineolaris; odorant-binding proteins; olfaction; real-time PCR; Plus-C OBP; classic OBP; alternative splice variants; next-generation sequencing
In insects, the perception and discrimination of odorants requires the involvement of odorant-binding proteins (OBPs). To gain a better molecular understanding of olfaction in the agronomic pest Lygus lineolaris (the tarnished plant bug), we used a transcriptomics-based approach to identify potential OBPs. In total, 33 putative OBP transcripts, including the previously reported Lygus antennal protein (LAP), were identified based on the characteristic OBP Cys signature and/or sequence similarity with annotated orthologous sequences. The L.lineolarisOBP (LylinOBP) repertoire consists of 20 classic' OBPs, defined by the spacing of six conserved Cys residues, and 12 Plus-C' OBPs, defined by the spacing of eight conserved Cys and one conserved Pro residue. Alternative splicing of OBP genes appears to contribute significantly to the multiplicity of LylinOBP sequences. Microarray-based analysis of chemosensory tissues (antennae, legs and proboscis) revealed enrichment of 21 LylinOBP transcripts in antennae, 12 in legs, and 15 in proboscis, suggesting potential roles in olfaction and gustation respectively. PCR-based determination of transcript abundance for a subset of the LylinOBP genes across multiple adult tissues yielded results consistent with the hybridization data.
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