期刊
INSECT MOLECULAR BIOLOGY
卷 21, 期 2, 页码 205-221出版社
WILEY
DOI: 10.1111/j.1365-2583.2011.01127.x
关键词
Drosophila; Lucilia; transcriptome; de novo assembly; alternative splice; transposable element
资金
- NIH [GM076051A2, RGM077618A, RGM076643A]
- College of Agriculture and Life Sciences at Texas AM University
- Texas AgriLife Research
- NSF [MCB-0951120]
- Palmerston North Medical Research Foundation
- Massey University
- College of Agriculture and Life Sciences at North Carolina State University
The blow fly Lucilia sericata (Diptera: Calliphoridae) (Meigen) is a nonmodel organism with no reference genome that is associated with numerous areas of research spanning the ecological, evolutionary, medical, veterinary and forensic sciences. To facilitate scientific discovery in this species, the transcriptome was assembled from more than six billion bases of Illumina and twenty-one million bases of 454 sequence derived from embryonic, larval, pupal, adult and larval salivary gland libraries. The assembly was carried out in a manner that enabled identification of putative single nucleotide polymorphisms (SNPs) and alternative splices, and that provided expression estimates for various life history stages and for salivary tissue. The assembled transcriptome was also used to identify transcribed transposable elements in L. sericata. The results of this study will enable blow fly biologists, dipterists and comparative genomicists to more rapidly develop and test molecular and genetic hypotheses, especially those regarding blow fly development and salivary gland biology.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据