期刊
INSECT MOLECULAR BIOLOGY
卷 17, 期 3, 页码 217-225出版社
WILEY
DOI: 10.1111/j.1365-2583.2008.00797.x
关键词
blood feeding; i-type lysozyme; mosquito
资金
- NIAID NIH HHS [R01 AI037083-09A2, R01AI037083, R01 AI037083] Funding Source: Medline
We sequenced and characterized two novel invertebrate-type lysozymes from the mosquito Anopheles gambiae. Alignment and phylogenetic analysis of these and a number of related insect proteins identified through bioinformatics strategies showed a high degree of conservation of this protein family throughout the Class Insecta. Expression profiles were examined for the two mosquito genes through semiquantitative and real-time PCR analysis. Lys i-1 transcripts were found in adult females in the fat body and Malpighian tubules, whereas Lys i-2 was detected only in fat bodies. Blood-feeding resulted in significantly increased transcript abundance for both genes in the midguts. Neither gene was upregulated following bacterial challenge.
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