期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 101, 期 -, 页码 47-56出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2018.07.006
关键词
Mode of action; Cry receptor; Sf21 cells; HEK293T cells; Heterologous expression
资金
- Generalitat Valenciana [GVPROMETEOII-2015-001, GVISIC2013-004]
- Spanish Ministry of Economy and Competitiveness [AGL2014-57752-C2-2-R]
- Spanish Ministry of Education, Culture and Sports [FPU15/05652]
- Japan Society for the Promotion of Science (JSPS) KAKENHI [15H02837, 15J08610]
- Grants-in-Aid for Scientific Research [15J08610] Funding Source: KAKEN
Cry proteins from Bacillus thuringiensis (Bt) have been used to control insect pests either as formulated sprays or as in Bt-crops. However, field-evolved resistance to Bt proteins is threatening the long-term use of Bt products. The SeABCC2 locus has been genetically linked to resistance to a Bt bioinsecticide (Xentarin (TM)) in Spodoptera exigua (a mutation producing a truncated form of the transporter lacking an ATP binding domain was found in the resistant insects). Here, we investigated the role of SeABCC2 in the mode of action of CrylAa, CrylAb, CrylAc, CrylCa, and two Cry1A-1Ca hybrids by expressing the receptor in Sf21 and HEK293T cell lines. Cell toxicity assays showed that Sf21 cells expressing SeABCC2 become susceptible to CrylA proteins. HEK293T cells expressing the transporter were found susceptible to CrylA proteins but not to CrylCa. The results with the Cry1A-1Ca hybrids suggest that domain II from CrylAb/c is crucial for the toxicity to Sf21 cells, whereas domain III from CrylAa/b is crucial for the toxicity to HEK293T cells. Binding assays showed that the CrylAc binding is of high affinity and specific to cells expressing the SeABCC2 transporter. Heterologous competition experiments support a model in which domain II of CrylAb/c has a common binding site in the SeABCC2 protein, whereas domain Ill of CrylAa/b binds to a different binding site in the SeABCC2 protein.
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