期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 43, 期 12, 页码 1142-1151出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2013.10.001
关键词
Olfaction; Cytochrome P450; Cytochrome b(5); NADPH-dependent cytochrome P450 reductase; Odorant clearance; Terpene
资金
- Genome Canada
- Genome British Columbia
- Genome Alberta
- Natural Sciences and Engineering Research Council (NSERC)
- University of British Columbia's Distinguished University Scholar program
The mountain pine beetle (MPB, Dendroctonus ponderosae Hopkins) is a significant pest of western North American pine forests. This beetle responds to pheromones and host volatiles in order to mass attack and thus overcome the terpenoid chemical defences of its host. The ability of MPB antennae to rapidly process odorants is necessary to avoid odorant receptor saturation and thus the enzymes responsible for odorant clearance are an important aspect of host colonization. An antenna-specific cytochrome P450, DponCYP345E2, is the most highly expressed transcript in adult MPB antenna. In in vitro assays with recombinant enzyme, DponCYP345E2 used several pine host monoterpenes as substrates, including (+)-(3)-carene, (+)-beta-pinene, ()-beta-pinene, (+)-limonene, (-)-limonene, (-)-camphene, (+)-alpha-pinene, ()-a-pinene, and terpinolene. The substrates were epoxidized or hydroxylated, depending upon the substrate. To complement DponCYP345E2, we also functionally characterized the NADPH-dependent cytochrome P450 reductase and the cytochrome b(5) from MPB. DponCYP345E2 is the first cytochrome P450 to be functionally characterized in insect olfaction and in MPB. (C) 2013 Elsevier Ltd. All rights reserved.
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