期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 42, 期 10, 页码 766-775出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2012.07.004
关键词
Bombyx mori; Cuticle-degrading enzyme; Entomopathogenic fungi; Melanization; Protease inhibitor
资金
- National Basic Research Program of China [2012CB114600]
- National Hi-Tech Research and Development Program of China [2011AA100306]
- National Natural Science Foundation of China [30972147]
- Graduate Technological Innovation Foundation of Southwest University of China [kb2010003]
Entomopathogenic fungi, such as Beauveria bassiana, penetrate the insect cuticle using a plethora of hydrolytic enzymes including cuticle-degrading proteases and chitinases, which are important virulence factors. The insect integument and hemolymph contains a relatively high concentration of protease inhibitors, which are closely involved with defense against pathogenic microorganisms. To elucidate the molecular mechanism underlying resistance against entomopathogenic fungi and to identify a new molecular target for improving fungal resistance in the silkworm, Bombyx mori, we cloned and expressed a novel silkworm TIL-type protease inhibitor BmSPI38, which was very stable over a wide range of temperatures and pH values. An activity assay suggested that BmSPI38 potently inactivated the insecticidal cuticle-degrading enzyme (CDEP-1) produced by B. bassiana and subtilisin A produced by Bacillus licheniformis. The melanization of sillcworm induced by CDEP-1 protease could also be blocked by BmSPI38. These results provided new insights into the molecular mechanisms whereby insect protease inhibitors provide resistance against entomopathogenic fungi, suggesting the possibility of using fungal biopesticides in sericulture. (c) 2012 Elsevier Ltd. All rights reserved.
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