期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 42, 期 8, 页码 537-544出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2012.04.001
关键词
Insect; Thrombosis; Plasminogen-activator; Fibrin(ogen)olytic
资金
- Chinese National Natural Science Foundation [30830021, 31000962, 31070701, 31025025]
- Ministry of Science and Technology [2010CB529800, 2009ZX09103-1/091]
- Ministry of Agriculture [2009ZX08009-159B]
The insect of Eupolyphaga sinensis Walker has been used as traditional anti-thrombosis medicine without bleeding risk for several hundreds years in eastern countries. Our previous work has identified a bifunctional anti-thrombosis protein containing both direct-acting fibrin(ogen)olytic and plasminogen-activating activities from the insect. By proteomics and transcriptome analysis, 105 serine proteases belonging to four families were identified from the ground beetle, E. sinensis and the classification is for serine proteases of this organism. Pharmacological test indicated that 5 (eupolytin 1-5) of them have the abilities to hydrolyze fibrin(ogen) and/or activate plasminogen. The current work revealed the extreme diversity of anti-thrombosis components in E. sinensis and anti-thrombosis molecular mechanisms of the traditional medicinal insect, and provided many templates for the development of new thrombolytic agents. Especially, these proteins, which contain both plasmin- and PA (plasminogen-activating)-like activities, are excellent candidates for anti-thrombosis medicines. (C) 2012 Elsevier Ltd. All rights reserved.
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