期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 41, 期 12, 页码 923-931出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2011.09.001
关键词
Alternative splicing; Chitin synthase; Insect; RNAi
资金
- National Key Project for Basic Research [2010CB126100]
- National Natural Science Foundation of China [31070715]
- National High Technology Research and Development Program of China [2011AA10A204]
- National Key Technology RD Program [2011BAE06B05]
- Fundamental Research Funds for the Central Universities [DUT11ZD113]
Insect chitin synthase A (CHSA) catalyzes chitin biosynthesis in tissues that develop from ectoderm. Since only one gene copy encodes CHSA, we hypothesized that CHSA is very likely to exist as isoforms through alternative splicing, and the functions of these isoforms may be tissue-specific. Besides the known alternative splicing exons in the mid-ORF region, we report here the alternative exons (OfCHSA-2a and OfCHSA-2b) of OfCHSA, the chitin synthase A from the lepidopteran pest Ostrinia furnacalis. Sequence analysis of the 5' upstream region of the transcription start site indicated that presences of two independent promoters for controlling the expression of OfCHSA-2a/b. Both OfCHSA-2a and OfCHSA-2b transcripts were preferentially expressed in the epidermis. During growth and development of O. furnacalis, OfCHSA-2a was mainly expressed during larval-larval molting and larval-pupal transformation, as well as in newly-laid eggs, while OfCHSA-2b was expressed only during the larval-larval molting. Gene silencing of OfCHSA-2a caused incomplete molting, while silencing of OfCHSA-2b exclusively influenced the head cuticle formation of the 3rd instar larval. Since O. furnacalis is phylogenetically close to the model insect Bombyx mori, the same undiscovered alternative splicing exon was also identified in BmCHSA by gDNA sequence alignment. This work may lead to greater understanding of the mechanism by which a single copy of the CHSA gene could fulfill various functions with tissue specificity. (C) 2011 Elsevier Ltd. All rights reserved.
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