期刊
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
卷 40, 期 2, 页码 138-145出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.ibmb.2010.01.005
关键词
Cry1B; Bacillus thuringiensis; ALP; GPI; Anthonomus grandis
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq - Brasil)
- Fundacao de Apoio A Pesquisa do Distrito Federal (FAPDF- Brasil)
Cry toxins from Bacillus thuringiensis (Bt) are used for insect control. They interact with specific receptors located on the host cell surface and are activated by host proteases following receptor binding resulting in midgut epithelial cells lysis. In this work we had cloned, sequenced and expressed a cry1Ba toxin gene from the B thuringiensis S601 strain which was previously shown to be toxic to Anthonomus grandis, a cotton pest. The Cry1Ba6 protein expressed in an acrystaliferous B. thuringiensis strain was toxic to A. grandis in bioassays. The binding of Cry1Ba6 toxin to proteins located in the midgut brush border membrane of A. grandis was analyzed and we found that Cry1Ba6 binds to two proteins (62 and 65 kDa) that showed alkaline phosphatase (ALP) activity. This work is the first report that shows the localization of Cry toxin receptors in the midgut cells of A. grandis. (C) 2009 Published by Elsevier Ltd.
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