期刊
INORGANICA CHIMICA ACTA
卷 361, 期 4, 页码 1116-1121出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.ica.2007.08.018
关键词
sensitised fluorescence; FRET; hemocyanin; sol-gel; oxygen sensor
Fluorescently labeled type-3 copper proteins have been proposed previously as solution oxygen sensors by using a FRET mechanism. Herein, we describe how this principle can be adapted to sense O-2 by means of proteins immobilized in optically transparent silica matrices. Specifically, the protein, hemocyanin from Octopus vulgaris N-terminally labeled with Cy5, is immobilized in two different kinds of optically transparent silica matrices, which appear to be a promising platform for enzyme encapsulation. The presented results provide proof of principle that fluorescently labeled proteins immobilized in a silica matrix can be implemented in a reusable, biocompatible and stable oxygen measuring device that might lead to new developments in the field of optical biosensing. (C) 2007 Elsevier B.V. All rights reserved.
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