[Ru(bpy)2(bppp)]2+ binds two different forms of the human telomeric G-quadruplex structure

A new complex [Ru(bpy)(2)(bppp)](2+) (bpy=2,2'-bipyridine, bppp=12-bromo-pyrido[2',3':5,6]pyrazino [2,3-f][1,10]phenanthroline) has been synthesized and characterized. The interaction of the title complex with two different forms of the human telomeric G-quadruplexes DNA was explored by means of CD spectroscopy. CD melting, fluorescent intercalator displacement (FID) and molecular docking studies. Results indicated that [Ru(bpy)(2)(bppp)](2+) not only induce formation of antiparallel G-quadruplex structure in the absence of metal cations, but also have the ability to stabilize the G-quadruplexes DNA. (c) 2012 Elsevier B.V. All rights reserved.