期刊
INORGANIC CHEMISTRY
卷 50, 期 19, 页码 9385-9392出版社
AMER CHEMICAL SOC
DOI: 10.1021/ic200986v
关键词
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资金
- National Science Foundation [CHE-0611944]
- National Institutes of Health [K99GM092970]
- NIH [1S10RR13886-01]
Fluorescent turn-on probes for nitric oxide based on seminaphthofluorescein scaffolds were prepared and spectroscopically characterized. The Cu(II) complexes of these fluorescent probes react with NO under anaerobic conditions to yield a 20-45-fold increase in integrated emission. The seminaphthofluorescein-based probes emit at longer wavelengths than the parent FL1 and FL2 fluorescein-based generations of NO probes, maintaining emission maxima between 550 and 625 nm. The emission profiles depend on the excitation wavelength; maximum fluorescence turn-on is achieved at excitations between 535 and 575 nm. The probes are highly selective for NO over other biologically relevant reactive nitrogen and oxygen species including NO3-, NO2-, HNO, ONOO-, NO2, OCl-, and H2O2. The seminaphthofluorescein-based probes can be used to visualize endogenously produced NO in live cells, as demonstrated using Raw 264.7 macrophages.
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