期刊
INORGANIC CHEMISTRY
卷 48, 期 16, 页码 7630-7638出版社
AMER CHEMICAL SOC
DOI: 10.1021/ic900247r
关键词
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资金
- Ministry of Education. Culture, Sports, Science and Technology (MEXT) of Japan [18310144, 18032045, 18033034, 18011005, 19036012, 19021028, 19651093]
- Council of Science and Technology Policy Coordination program of Science and Technology Projects
- Novartis Foundation for the Promotion of Science
- Shimadzu Science Foundation
- Kato Memorial Bioscience Foundation
- Astellas Foundation for Research
- Memorial Foundation
- Terumo Life Science Foundation
- Nagase Science and technology Foundation
- Asahi Glass Foundation
- Cosmetology Research Foundation
- Grants-in-Aid for Scientific Research [18310144, 18032045, 19651093, 19036012, 18011005, 18033034, 19021028] Funding Source: KAKEN
The physiological roles of free Zn2+ have attracted great attention. To clarify those roles, there has been a need for ratiometric fluorescent Zn2+ probes for practical use. We report the rational design and synthesis of a series of ratiometric fluorescent Zn2+ probes. The structures of the probes are based on the 7-hydroxycoumarin structure. We focused on the relationship between the electron-donating ability of the 7-hydroxy group and the excitation spectra of 7-hydroxycoumarins, and exploited that relationship in the design of the ratiometric probes; as a result, most of the synthesized probes showed ratiometric Zn2+ -sensing properties. Then, we designed and synthesized ratiometric Zn2+ probes that can be excited with visible light, by choosing adequate substituents on coumarin dyes. Since one of the probes could permeate living cell membranes, we introduced the probe to living RAW264 cells and observed the intracellular Zn2+ concentration via ratiometric fluorescence microscopy. As a result, the ratio value of the probe changed quickly in response to intracellular Zn2+ concentration.
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