期刊
INNATE IMMUNITY
卷 21, 期 5, 页码 504-511出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425914551074
关键词
Airway epithelia; cellular differentiation; human embryonic stem cells; innate immune response; TNF-alpha
资金
- CIHR Emerging Team Grant
- CIHR-Banting Fellowship
The acquisition of innate immune response is requisite to having bona fide differentiation of airway epithelium. Procedures developed to differentiate lung airway from human pluripotent stem cells (hPSCs) have demonstrated anecdotal evidence for innate immune response, but an in-depth exploration of response levels is lacking. Herein, using an established method of airway epithelial generation from hPSCs, we show that hPSC-derived epithelial cells are able to up-regulate expression of TNF, IL8 and IL1 in response to challenge with bacterial endotoxin LPS, but lack response from genes associated with innate immune response in other cell types. Further, stimulation of cells with TNF- resulted in auto-induction of TNF transcript, as well as cytokine responses of IL8 and IL1. The demonstration of innate immune induction in hPSC-derived airway epithelia gives further strength to the functionality of invitro protocols aimed at generating differentiated airway cells that can potentially be used in a translational setting. Finally, we propose that innate immune challenge of airway epithelium from human pluripotent stem cell sources be used as a robust validation of functional invitro differentiation.
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