期刊
INNATE IMMUNITY
卷 18, 期 1, 页码 55-69出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425910392934
关键词
Cervical cancer; integrins; Toll-like receptors
资金
- Northern Cancer Research Foundation (Thunder Bay, ON)
- National Science and Engineering Research Council (NSERC)
We hypothesized that development of cervical cancer is associated with alterations in the expression of innate immune receptors, i.e. integrins and TLRs, and that these alterations can be induced by infectious agents. We have studied the expression of these proteins in cervical biopsy tissues and cervical cancer-derived cell lines HeLa, CaSki, SiHa, C-33 A, and ME180. Immunohistochemistry analysis demonstrated an increase in integrin alpha v, beta 3, beta 4, and beta 6 expression in the epithelium during the development of cervical cancer. A clear trend towards higher expression of integrin beta 6 in cell lines harbouring human papillomavirus (HPV) genetic material, compared to HPV-negative C-33 A, was observed. To investigate whether bacterial infection can alter the expression of TLRs and integrins, we infected HeLa cells by two pathogens, Escherichia coli and Pseudomonas aeruginosa, using a common bacterium of the female genital tract, Lactobacillus reuteri, as a control. Infection with E. coli or P. aeruginosa, but not with L. reuteri, significantly altered the expression of TLR and integrins, particularly of TLR4 and integrin beta 6. Considering that both integrin beta 6 and TLR4 play important roles in tumorigenesis, our data suggest that bacterial infection may trigger cancer development in HPV-infected cervical epithelium.
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