期刊
INNATE IMMUNITY
卷 14, 期 1, 页码 13-24出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425907087349
关键词
folimycin; LPS; nitric oxide; TLR4
资金
- Director of Indian Institute of Science [2A, 191/MCB]
- Department of Atomic energy [DAEO 119]
- ICMR Center for Medical Microbiology
- DBT
Lipopolysaccharide (LPS) is a major cell wall component of Gram-negative bacteria and signals through a receptor complex which consists of TLR4, MD-2 and CD14. LPS signaling in macrophages induces the production of many pro-inflammatory molecules, including nitric oxide (NO). In this study, we have shown that folimycin, a macrolide antibiotic and a specific inhibitor of vacuolar ATPase (V-ATPase), inhibits LPS-induced NO production, but not TNF-alpha alpha production, in murine elicited peritoneal macrophages. However, folimycin did not affect interferon-gamma induced NO production. LPS-induced iNOS mRNA and protein expression and NF-kappa B activation were also inhibited by folimycin. Interestingly, folimycin-treated cells showed reduced surface expression of TLRA molecules and dilated Golgi apparatus. These findings suggest that folimycin, by inhibiting V-ATPases, alters intra-Golgi pH, which in turn causes defective processing and reduced surface expression of TLR4 reducing the strength of LPS signaling in murine macrophages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据