期刊
INHALATION TOXICOLOGY
卷 22, 期 -, 页码 37-47出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/08958378.2010.518377
关键词
Particulate matter; biological/toxicological effects; standard reference materials; PM2.5; oxidative stress; inflammation; cytotoxicity; chemical composition; redox activity; IL-8 adsorption
类别
资金
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Institutes of Health Research (CIHR)
- AllerGen Network of Centres of Excellence [07-A5]
- Canada Foundation for Innovation (CFI)
- Ontario Research Fund (ORF)
The composition of airborne particulate matter (PM) varies widely depending on its source, and recent studies have suggested that particle-associated adverse health effects are related to particle composition. The objective of this study was to compare the biological/toxicological effects of different source-related PM. Specifically, we investigated the biological/toxicological effects of standard reference materials (SRMs): non-ferrous dust (PD-1, industrial), urban PM (UPM, SRM1648a), and diesel PM (DPM, SRM2975), and ambient PM2.5 (PM with an aerodynamic diameter <2.5 mu m) collected at an urban site (Toronto, Canada). The dithiothreitol assay was used to measure the redox activity of the particles. Human alveolar epithelial cells (A549) were exposed to a range of concentrations (10-1000 mu g/ml) of total PM, and the respective water-soluble and insoluble fractions, for 24 h. Biological responses were then evaluated in terms of cytotoxicity and interleukin (IL)-8 release, and compared with the PM composition and redox activity. We demonstrated that transition metal-enriched PD-1 exhibited the greatest cytotoxic effect (LD50 values of 100-400 mu g/ml vs. >1000 mu g/ml for the SRM1648a, SRM2975, and ambient PM2.5). Similarly, the PM-induced release of IL-8 was greatest for PD-1 (similar to 6-9 ng/ml vs. similar to 1.5-3 ng/ml for others). These endpoints were more responsive to metals as compared with compared with secondary inorganic ions and organic compounds. Interestingly, we demonstrated a high degree of adsorption of IL-8 to the various SRMs and ambient PM2.5, and subsequently derived a new correction method to aid in interpretation of these data. These characteristics likely impart differential effects toward the toxic and immune effects of PM.
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