期刊
INFLAMMATORY BOWEL DISEASES
卷 20, 期 5, 页码 861-871出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/MIB.0000000000000023
关键词
short-chain fatty acids; enteral nutrition; Crohn's disease; pediatrics; gut microbiota
资金
- Greek State Scholarship Foundation
- Hellenic Foundation of Gastroenterology, and Nutrition
- Barr Endowment Fund
- Yorkhill Children's Foundation
- Medical Research Council patient research cohorts initiative grant [G0800675]
- NHS Research Scotland career fellowship award
- Catherine McEwan Foundation
- Yorkhill IBD fund
- Crohn's in Childhood Research Association (CICRA)
- MSD Immunology
- Abbott
- Dr Falk
- Nestle
- Ferring Pharmaceuticals
- Falk
- ILSI Europe
- Foods Standards Agency
- Warner Chillcott
- MSD
- Ferring
- MRC [G0800675] Funding Source: UKRI
- Medical Research Council [G0800675] Funding Source: researchfish
- Medical Research Foundation [C0482] Funding Source: researchfish
Background: The gut microbiota is implicated in the pathogenesis of Crohn's disease (CD). Exclusive enteral nutrition (EEN) is a successful treatment, but its mode of action remains unknown. This study assessed serial changes in the fecal microbiota milieu during EEN. Methods: Five fecal samples were collected from CD children: 4 during EEN (start, 15, 30, end EEN approximately 60 days) and the fifth on habitual diet. Two samples were collected from healthy control subjects. Fecal pH, bacterial metabolites, global microbial diversity abundance, composition stability, and quantitative changes of total and 7 major bacterial groups previously implicated in CD were measured. Results: Overall, 68 samples were from 15 CD children and 40 from 21 control subjects. Fecal pH and total sulfide increased and butyric acid decreased during EEN (all P < 0.05). Global bacterial diversity abundance decreased (P < 0.05); a higher degree of microbiota composition stability was seen in control subjects than in CD children during EEN (at P <= 0.008). Faecalibacterium prausnitzii spp concentration significantly decreased after 30 days on EEN (P < 0.05). In patients who responded to EEN, the magnitude of the observed changes was greater and the concentration of Bacteroides/Prevotella group decreased (P < 0.05). All these changes reverted to pretreatment levels on free diet, and EEN microbiota diversity increased when the children returned to their free diet. Conclusions: EEN impacts on gut microbiota composition and changes fecal metabolic activity. It is difficult to infer a causative association between such changes and disease improvement, but the results do challenge the current perception of a protective role for F. prausnitzii in CD.
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