期刊
INFLAMMATORY BOWEL DISEASES
卷 19, 期 13, 页码 2848-2856出版社
OXFORD UNIV PRESS INC
DOI: 10.1097/01.MIB.0000435444.14860.ea
关键词
GPR43; SCFAs; cytokine; lymphocyte; colitis
资金
- General Incorporated Foundation from Aichi Medical University Aikeikai
- Research Organization for Gastro-Enterological Disease Treatment (NPO-ROGET)
- Daiich Sankyo Co., Ltd.
- Astra Zeneca Co., Ltd.
Background:Short-chain fatty acids (SCFAs), which are produced by the fermentation of dietary fiber by intestinal microbiota, may positively influence immune responses and protect against gut inflammation. SCFAs bind to G protein-coupled receptor 43 (GPR43). Here, we show that SCFA-GPR43 interactions profoundly affect the gut inflammatory response.Methods:Colitis was induced by adding dextran sulfate sodium to the drinking water of GPR43 knockout (-/-) and wild-type mice.Results:Dextran sulfate sodium-treated GPR43(-/-) mice exhibited weight loss, increased disease activity index (a combined measure of weight loss, rectal bleeding, and stool consistency), decreased hematocrit, and colon shortening, resulting in significantly worse colonic inflammation than in wild-type mice. Tumor necrosis factor alpha and interleukin 17 protein levels in the colonic mucosa of GPR43(-/-) mice were significantly higher than in wild-type mice. Treatment of wild-type mice with 150 mM acetate in their drinking water markedly improved these disease indices, with an increase in colon length and decrease in the disease activity index; however, it had no effect on GPR43(-/-) mice. Mononuclear cell production of tumor necrosis factor alpha after lipopolysaccharide stimulation was suppressed by acetate. This effect was inhibited by anti-GPR43 antibody.Conclusions:SCFA-GPR43 interactions modulate colitis by regulating inflammatory cytokine production in mononuclear cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据