期刊
INFLAMMATORY BOWEL DISEASES
卷 16, 期 9, 页码 1557-1566出版社
OXFORD UNIV PRESS INC
DOI: 10.1002/ibd.21224
关键词
ulcerative colitis; spermine oxidase; polyamines; gene expression; immunohistochemistry; disease activity index
资金
- P & G Pharmaceuticals
- National Institutes of Health (NIH) [R01AT004821, 3R01AT004821-02S1, R01DK053620, R01CA051085, R01CA098454, K08DK080221, 5T32DK007673]
- NIH (Vanderbilt Digestive Disease Research Center) [P30DK058404]
Background: Polyamines are important in cell growth and wound repair, but have also been implicated in inflammation-induced carcinogenesis. Polyamine metabolism includes back-conversion of spermine to spermidine by the enzyme spermine oxidase (SMO), which produces hydrogen peroxide that causes oxidative stress. In ulcerative colitis (UC), levels of spermine are decreased compared to spermidine. Therefore, we sought to determine if SMO is involved in UC. Methods: Colon biopsies and clinical information from subjects undergoing colonoscopy for evaluation of UC or colorectal cancer screening were utilized from 16 normal controls and 53 UC cases. Histopathologic disease severity was graded and the Mayo Disease Activity Index (DAI) and endoscopy subscore assessed. SMO mRNA expression was measured in frozen biopsies by Taq-Man-based real-time polymerase chain reaction (PCR). Formalin-fixed tissues were used for SMO immunohistochemistry. Results: There was a 3.1-fold upregulation of SMO mRNA levels in UC patients compared to controls (P = 0.044), and a 3.7-fold increase in involved left colon versus paired uninvolved right colon (P < 0.001). With worsening histologic injury in DC there was a progressive increase in SMO staining of mononuclear inflammatory cells. There was a similar increase in SMO staining with worsening endoscopic disease severity and strong correlation with the DAI (r = 0.653, P < 0.001). Inflammatory cell SMO staining was increased in involved left colon versus uninvolved right colon. Conclusions: SMO expression is upregulated m DC tissues. deriving from increased levels in mononuclear inflammatory cells. Dysregulated polyamine homeostasis may contribute to chronic DC by altering immune responses and increasing oxidative stress.
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