期刊
INFLAMMATORY BOWEL DISEASES
卷 15, 期 7, 页码 1022-1031出版社
OXFORD UNIV PRESS INC
DOI: 10.1002/ibd.20900
关键词
intestinal inflammation; animal models; IL-10 deficient mice; Bifidobacterium animalis
资金
- NCRR NIH HHS [P40 RR018603-02, P40 RR018603] Funding Source: Medline
- NIDDK NIH HHS [R01 DK53347, P30 DK034987, R01 DK053347-10A1, T32 DK007737, P30 DK 034987, R01 DK053347] Funding Source: Medline
- NIGMS NIH HHS [GM 00678, K12 GM000678] Funding Source: Medline
- PHS HHS [P40 R018603] Funding Source: Medline
Background: We recently showed that Bifidobacterium animalis is more prevalent within the colons of interleukin (IL)-10-deficient (-/-) mice than in wildtype (WT) animals colonized with the same specific pathogen-free (SPF) fecal contents. Here we tested the ability of this organism to cause T-cell-inediated intestinal inflammation by introducing it into germ-free (GF) IL-10-/- mice. Methods: GF IL-10-/- or WT mice were monoassociated with Bifidobacterium animalis subsp. animalis ATCC (American Type Culture Collection, Manassas, VA) 25527T or with B. infantis ATCC 15697 T. Inflammation was measured by blinded histologic scores of the duodenum, cecum, and colon and by spontaneous secretion of IL-12/IL-23 p40 from colonic explants. Bacterial antgen-specific CD4(+) mesenteric lymph node (MLN) T-cell recall responses were measured in response to antigen-presenting cells (APC) pulsed with bacterial lysates. Results: B. animalis caused marked duodenal inflammation and mild colitis in monoassociated IL-10-/- mice, whereas the intestinal tracts of WT animals remained free of inflammation. B. infantis colonization resulted in mild inflammation in the duodena of IL-10-/- mice. CD4(+) MLN T cells from B. animalis monoassociated IL-10-/- mice secreted hi-h levels of IFN-gamma and IL-17 in response to B. animalis lysate. B. animalis equally colonized the different intestinal regions of WT and IL-10-/- mice. Conclusions: B. animalis, a traditional probiotic species that is expanded in experimental colitis in this model, induces marked duodenal and mild colonic inflammation and TH1/TH17 immune responses when introduced alone into GF IL-10-/- mice. This Suggests a potential pathogenic role for this commensal bacterial species in a Susceptible host. (Inflamm Bowel Dis 2009:15:1022-1031)
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