期刊
INFLAMMATORY BOWEL DISEASES
卷 15, 期 6, 页码 872-882出版社
JOHN WILEY & SONS INC
DOI: 10.1002/ibd.20860
关键词
Crohn's disease; Escherichia coli; adherent-invasive Escherichia coli; ecologic parameters; seropathotyping
资金
- Spanish Ministry of Education and Science [SAF2006-00414]
- Spanish Ministry of Health and Consumer Affairs [REIPI RD06/0008-1018]
Background: Escherichia coli, particularly the adherent-invasive E. coli (AIEC) pathovar, has been increasingly implicated in the ethiopathogenesis of Crohn's disease (CD). We describe the richness, abundance, diversity, and pathogenic features of E. coli and AIEC strains that colonize the intestinal mucosa. Methods: Approximately 100 E. coli colonies per biopsy from 20 CID patients (18 biopsies from colon and 23 from ileum) and 28 healthy controls (C) (25, colon; 27, ileum) were isolated. Repetitive extragenic palindrome-polymerase chain reaction (Rep-PCR) and pulsed field gel electrophoresis (PFGE) were used to analyze the clonality of isolates. For AIEC identification, adhesion and invasion assays were performed over Intestine-407 cells, and the capacity to survive and replicate intracellularly was determined over macrophages J774. The serotypes, phylotypes, and genotypes (19 virulence genes) of strains were also investigated. Results: Mucosa-associated E. coli richness (E. coli subtypes/ patient: C = 2.0 +/- 1.0 CD = 2.1 +/- 1.3) and diversity (Shannon Index: H'(C): 2.1 +/- 0.6; H'(CD): 2.5 +/- 0.8) were similar between CD and C, but higher E. coli counts were characteristic of CD patients (P = 0.010), particularly those with Crohn's ileitis (P = 0.001). Host-specific pulsotypes shared virulence features of ExPEC at similar frequencies between CID and C, except for iucD, which was more prevalent in E. coli from controls (C: 75%, CD: 40%, P = 0.027). In contrast, greater AIEC prevalence (% subjects with AIEC: CD = 51.9%; C = 16.7%; P = 0.003). abundance (% AIEC/E. coli: CD = 3.8 +/- 5.0%; C = 1.5 +/- 3.8%; P = 0.039) and richness (number of AIEC subtypes: CD = 0.8 +/- 1.4; C = 0.2 +/- 0.4; P = 0.015) of E. coli strains belonging to the AIEC pathovar was observed for CD patients. AIEC subtypes showed a high variability of seropathotypes and pulsotypes, although the 132 phylogroup was the most prevalent (AIEC: 64%, non-AIEC: 38%, P = 0.044). Conclusions: New data about ecological parameters of AIEC reinforces the implication of AIEC in CD.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据