期刊
INFLAMMATION RESEARCH
卷 60, 期 3, 页码 245-253出版社
SPRINGER BASEL AG
DOI: 10.1007/s00011-010-0260-y
关键词
Drug delivery systems; Drug targeting; Liposomes; Acute lung injury; Endothelial cells
资金
- Bavarian Research Fund
Cationic liposomes have been shown to target angiogenic endothelial cells in lungs and joints with evidence of chronic inflammation. We sought to determine whether cationic liposomes accumulate in acutely inflamed lung tissue. Acute lung injury was induced by intratracheal instillation of lipopolysaccharide (LPS) in Sprague Dawley rats. The controls received saline. Following instillation, the rats were ventilated for 5 h. Four hours after LPS-instillation each rat received rhodamine-labeled, cationic liposomes intravenously. The liposomes were allowed to circulate for 1 h. Thereafter, a bronchoalveolar lavage (BAL) was done and the lungs were perfused with saline and formalin. Accumulation of liposomes was assessed by quantitative confocal microscopy and determination of rhodamine-content in lung tissue. LPS induced a significant increase in BAL white blood cell count (3,444 +/- A 1,420 vs. 1,314 +/- A 906*10(3)/mu l) and cytokines (IL-1 beta: 145.57 vs. 51.94 pg/ml; TNF-alpha: 3,467.5 vs. 42.1 pg/ml) as compared to controls. Cationic liposomes exhibited an accumulation up to twofold in the inflamed lung tissue as compared to healthy lungs (fluorescent pixels 2.93 vs. 1.90(%)). Our findings indicate that cationic liposomes accumulate in the acutely inflamed lung tissue. This uptake raises the possibility of using cationic liposomes to direct diagnostic/therapeutic agents selectively to the sites of acute inflammation in the lung.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据