期刊
INFLAMMATION
卷 37, 期 6, 页码 2174-2189出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-014-9951-9
关键词
mast cells; Candida albicans; fungicidal activity; phagosome
资金
- Marie Curie Early Stage Research Training Fellowship of the European Community's Sixth Framework Programme [504926]
- University of Trieste
- Foundation Foreman Casali (Trieste, Italy)
- Consorzio per lo Sviluppo Internazionale dell'Universita degli Studi di Trieste (CSIUT)
Mast cells (MCs) reside in tissues that are common targets of Candida spp. infections, and can exert bactericidal activity, but little is known about their fungicidal activity. MCs purified from rat peritoneum (RPMC) and a clinical isolate of C. albicans, were employed. Ingestion was evaluated by flow cytometry (FACS) and optical microscopy. The killing activity was assayed by FACS analysis and by colony forming unit method. RPMC degranulation was evaluated by beta-hexosaminidase assay and phosphatidylserine externalization by FACS. Phagocytosing RPMC were also analyzed by transmission electron microscopy. Herein, we show that the killing of C. albicans by RPMC takes place in the extracellular environment, very likely through secreted granular components. Ultrastructural analysis of the ingestion process revealed an unusual RPMC-C. albicans interaction that could allow fungal survival. Our findings indicate that MCs have a positive role in the defense mechanism against Candida infections and should be included among the cell types involved in host-defense against this pathogen.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据