期刊
INFLAMMATION
卷 35, 期 5, 页码 1669-1675出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10753-012-9483-0
关键词
cytokines; stevioside; nuclear factor-kappaB (NF-kappa B); mitogen-activated protein kinase (MAPK); RAW264.7 cells
资金
- National Natural Science Foundation of China [30972225, 30771596]
- Research Fund for the Doctoral Program of Higher Education of China [20110061130010]
Stevioside, a diterpene glycoside isolated from Stevia rebaudiana, has been reported to have anti-inflammatory properties. However, the underlying molecular mechanisms are not well understood. The objective of this study was to investigate the molecular mechanism of stevioside in modifying lipopolysaccharide (LPS)-induced signal pathways in RAW264.7 cells. RAW264.7 cells were stimulated with LPS in the presence or absence of stevioside. The expression of pro-inflammatory cytokines was determined by enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction. Nuclear factor-kappa B (NF-kappa B), inhibitory kappa B (I kappa B alpha) protein, p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK) were determined by western blot. The results showed that stevioside dose-dependently inhibited the expression of tumor necrosis factor-alpha, interleukin-6, and interleukin-1 beta in LPS-stimulated RAW264.7 cells. Western blot analysis showed that stevioside suppressed LPS-induced NF-kappa B activation, I kappa Ba degradation, phosphorylation of ERK, JNK, and P38. Our results suggest that stevioside exerts an anti-inflammatory property by inhibiting the activation of NF-kappa B and mitogen-activated protein kinase signaling and the release of proinflammatory cytokines. These findings suggest that stevioside may be a therapeutic agent against inflammatory diseases.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据