期刊
INFECTION GENETICS AND EVOLUTION
卷 20, 期 -, 页码 330-335出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.meegid.2013.09.015
关键词
Astrovirus; Epidemiology; Genotyping; Viral gastroenteritis; Italy
资金
- grant MicroMap [PON01_02589]
- grant Sorveglianza epidemiologica e molecolare delle gastroenteriti acute ad eziologia virale, Ricerca Finalizzata Ministero della Salute
Human astroviruses (HAstVs) are important enteric pathogens and can be classified genetically and antigenically into eight types. During surveillance of HAstVs in Italy, type-4 HAstVs were detected only sporadically and found to cluster into two distinct genetic groups. Upon sequence analysis of the 30 end of the polymerase gene (ORF1b) and of the full-length ORF2, the 2008 type-4 HAstV strains were characterised as a novel ORF2 genetic lineage, designated as 4c. The 2008 type-4 HAstVs also shared the ORF1b gene with similar HAstV-4c strains detected globally, thus displaying a conserved ORF1b/ORF2 asset. By interrogation of the databases, this novel lineage 4c accounted for 60.8% of the type-4 strains identified worldwide and the vast majority of recent type-4 HAstVs. The 2002 type-4 HAstVs displayed a type-4b ORF2, whereas in the ORF1b they resembled type-1 HAstVs. This inconsistency suggests a possible recombinant origin, with the RNA switch taking place upstream the ORF1b/ORF2 junction region. Also, recombination likely played a role in the diversification of the ORF2 of the three type-4 lineages. Multi-target analysis is required for appropriate characterisation and identification of recombinant HAstVs. (C) 2013 Elsevier B. V. All rights reserved.
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