期刊
INFECTION GENETICS AND EVOLUTION
卷 11, 期 8, 页码 2103-2105出版社
ELSEVIER
DOI: 10.1016/j.meegid.2011.08.029
关键词
S. typhi; Chronic typhoid carriers; Nested PCR; Liver; Gallbladder
It is suggested that Salmonella typhi resides mostly in hepatobiliary system especially in gallbladder in chronic typhoid carriers. It is not very clear whether in gallbladder lumen or on its wall or in liver. However, we had been successful in detecting S. typhi in liver by PCR targeting flagellin gene sequences. Therefore, in the present study, we tried to isolate the bacterium from liver tissue collected from dead bodies brought for post mortem examination. We could isolate S. typhi in 2 of 20 such liver tissues examined by using conventional isolation techniques. The isolates were identified by routine phenotypic characters and were confirmed by amplification and sequencing of two conserved genes i.e. 165 rDNA and flagellin (fliC) gene followed by blasting on www.ncbi.nlm.nih.gov. (C) 2011 Elsevier B.V. All rights reserved.
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