期刊
INFECTION GENETICS AND EVOLUTION
卷 11, 期 6, 页码 1250-1255出版社
ELSEVIER
DOI: 10.1016/j.meegid.2011.04.014
关键词
Human African trypanosomiasis; Trypanosoma brucei gambiense; Seropositive; Cote d'Ivoire; Guinea; Human reservoir; Control strategies
资金
- Institut de Recherche pour le Developpement (IRD)
- Ministere Francais des Affaires Etrangeres (Fonds de Solidarite Prioritaire Recherches en Entomologie, Formation et Strategies de formation, le cas du paludisme et de la Trypanosomose Humaine Africaine)
- World Health Organisation (WHO)
Thanks to its sensitivity and its ease of use in the field, the card agglutination test for trypanosomiasis (CATT) is widely used for serological screening of Trypanosoma brucei gambiense human African trypanosomiasis (HAT). Positive subjects are then examined by microscopy to confirm the disease. However, the CATT exhibits false-positive results raising the question of whether CATT-positive subjects who are not confirmed by microscopic detection of trypanosomes (SERO) are truly exposed to T.b. gambiense infection. For this purpose, we applied microsatellite genotyping on DNA extracted from blood of both HAT confirmed patients and SERO subjects in Guinea and Cote d'Ivoire since microsatellite genotyping has proved useful for the study of T.b. gambiense genetic diversity. Problems of amplification failures raise the question of the sensitivity of microsatellite markers when applied on biological samples especially from SERO subjects for who low blood parasitaemia are suspected. Nevertheless, we have shown that the trypanosomes from SERO individuals that have been genotyped belong to T.b. gambiense group 1 and were identical to those found in HAT patients. These results constitute the first evidences that at least some SERO are indeed infected by T.b. gambiense group 1 and that they may constitute a human reservoir of parasite in HAT foci. Whether these individuals should undergo treatment remains an open question as long as their role in HAT transmission is unknown. Our results strongly recommend the follow-up of such subjects to improve control strategies. (C) 2011 Elsevier B.V. All rights reserved.
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