期刊
INFECTION GENETICS AND EVOLUTION
卷 8, 期 6, 页码 825-834出版社
ELSEVIER
DOI: 10.1016/j.meegid.2008.08.008
关键词
Parallel substitution; Experimental evolution; Fitness; Host specificity; Virus capsid
资金
- National Institute of Health [P20 RR16448]
Genetic variation in viral structural proteins is often explained by evolutionary escape of strong host defenses through processes such as immune evasion, host switching, and tissue tropism. An understanding of the mechanisms driving evolutionary change in virus surface proteins is key to designing effective intervention strategies to disease emergence. This Study investigated the predictability of virus genomic evolution in response to highly specific differences in host receptor structure. The bacteriophage Phi X 174 was evolved on three E. coli mutant hosts, each differing only by a single sugar group in the lipopolysaccharides, used for phage attachment. Large phage populations were used in order to maximize the amount of sequence space explored by mutation, and thus the potential for parallel evolution. Repeatability was assessed by genome sequencing of multiple isolates from endpoint Populations and by fitness of the endpoint population relative to its ancestor. Evolutionary lines showed similar magnitudes of fitness increase between treatments. Only one mutation, occurring in the internal DNA pilot protein H, was completely repeatable, and it appeared to be a necessary stepping stone toward further adaptive change. Substitutions in the surface accessible major capsid protein F appeared to be involved in capsid stability rather than specific interactions with host receptors, suggesting that nonspecific alterations to capsid structure could be an important component of adaptation to novel hosts. 33% of mutations were synonymous and showed evidence of selection oil codon usage. Lastly, results supported previous findings that evolving populations of small ssDNA viruses may maintain relatively high levels of genetic variation. (C) 2008 Published by Elsevier B.V.
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