期刊
INFECTION GENETICS AND EVOLUTION
卷 8, 期 2, 页码 213-216出版社
ELSEVIER
DOI: 10.1016/j.meegid.2007.11.006
关键词
cysticercosis; experimental infections; genetic variability; RAPD; swine; Taenia solium
Taenia solium cysticerci recovered from naturally infected pigs from Mexico, Honduras and Tanzania show a clonal structure and local lineages with probable events of genetic recombination without genetic flow within them, as revealed by RAPD. To evaluate genetic polymorphism from cysticerci recovered from experimental infections, 4 pigs were infected with T solium eggs obtained from tapeworms released by 3 human carriers, a 10-year-old female, a 25-year-old female, and a 44-year-old male, the 4th pig was infected with a mixture of eggs from the 3 tapeworms. Each pig was orally inoculated with 50,000 eggs. After 16 weeks pigs were humanely euthanized and cysticerci were excised. Parasites recovered from each pig were analyzed by RAPD. The proportion of polymorphic loci and the mean heterozygosity as well as a dendogram and an analysis of principal coordinate and minimum spanning tree were obtained. All four pigs developed viable cysticerci; the percent infection was obtained from the ratio of the number of eggs used for infection and the number of cysticerci counted in each pig after necropsy. Infection varied from 0.2 to 4.2%. The values obtained for the proportion of polymorphic loci (0.14-0.55) and the average of expected heterozygosity (0.06-0.22) in the present experimental infection had a broader range than those reported in the literature from natural infections. The dendogram obtained clustered cysticerci into two main groups; the minimum spanning tree allowed to corroborate the data obtained in the dendogram and gave a better discrimination because in a three-dimensional plot it was easier to see that all cysticerci from each tapeworm were clustered amongst themselves. The results obtained could be hypothetically explained because environmental factors and genetic selection agents present in nature influence natural infections but do not participate in experimental ones. (C) 2007 Elsevier B.V. All rights reserved.
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