期刊
INFECTION AND IMMUNITY
卷 81, 期 4, 页码 1267-1276出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.00864-12
关键词
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资金
- National Institutes of Health [5P20RR016463, 8P20GM103423, 2R01AI054928, 2R01DE017088]
- National Center for Advancing Translational Sciences [UL1TR000124]
- National Center for Research Resources [5P20RR016463]
- National Institute of General Medical Sciences from the National Institutes of Health [8P20 GM103423]
- American Society for Microbiology
- Howard Hughes Medical Institute
Candida albicans causes both mucosal and disseminated infections, and its capacity to grow as both yeast and hyphae is a key virulence factor. Hyphal formation is a type of polarized growth, and members of the SR (serine-arginine) family of RNA-binding proteins influence polarized growth of both Saccharomyces cerevisiae and Aspergillus nidulans. Therefore, we investigated whether SR-like proteins affect filamentous growth and virulence of C. albicans. BLAST searches with S. cerevisiae SR-like protein Npl3 (ScNpl3) identified two C. albicans proteins: CaNpl3, an apparent ScNpl3 ortholog, and Slr1, another SR-like RNA-binding protein with no close S. cerevisiae ortholog. Whereas ScNpl3 was critical for growth, deletion of NPL3 in C. albicans resulted in few phenotypic changes. In contrast, the slr1 Delta/Delta mutant had a reduced growth rate in vitro, decreased filamentation, and impaired capacity to damage epithelial and endothelial cells in vitro. Mice infected intravenously with the slr1 Delta/Delta mutant strain had significantly prolonged survival compared to that of mice infected with the wild-type or slr1 Delta/Delta mutant complemented with SLR1 (slr1 Delta/Delta+SLR1) strain, without a concomitant decrease in kidney fungal burden. Histopathology, however, revealed differential localization of slr1 Delta/Delta hyphal and yeast morphologies within the kidney. Mice infected with slr1 Delta/Delta cells also had an increased brain fungal burden, which correlated with increased invasion of brain, but not umbilical vein, endothelial cells in vitro. The enhanced brain endothelial cell invasion was likely due to the increased surface exposure of the Als3 adhesin on slr1 Delta/Delta cells. Our results indicate that Slr1 is an SR-like protein that influences C. albicans growth, filamentation, host cell interactions, and virulence.
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