期刊
INFECTION AND IMMUNITY
卷 79, 期 5, 页码 2043-2050出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.01086-10
关键词
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资金
- 3R Research Foundation, Switzerland [107-07]
- Friends of the Pasteur Institute of Brussels
- Belgian Federal Public Service [RT-06/5-Alteval-3]
We investigated the early innate immune responses induced in human intestinal epithelial cells (IEC) by the three defined Toxoplasma gondii genotype strains. Transcriptome analysis revealed that among differentially expressed genes, beta-defensins distinguished the most IEC infected by fast-or slow-replicating T. gondii genotypes. Although beta-defensin 1 and 3 genes were not expressed in host cells at early time points postinfection, the slow-replicating type II and III parasites induced high levels of beta-defensin 2 gene expression. Notably, no beta-defensin 2 gene expression occurred early after infection with the fast-replicating type I parasite. However, activation of this gene in IEC by poly(I:C) treatment prior to infection substantially decreased parasite viability, and pretreatment of parasites with synthetic beta-defensin 2 significantly reduced their infectivity of IEC. These findings strongly support the modulation of early beta-defensin 2 expression as a mechanism used by type I T. gondii parasites to mediate immune evasion.
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