期刊
INFECTION AND IMMUNITY
卷 78, 期 11, 页码 4936-4943出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/IAI.01118-09
关键词
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资金
- Heartland of the American Heart Association [0660072Z]
- NIH [AI077782, AI061691]
- National Center for Research Resources [RR-P20 RR017686]
- Terry C. Johnson Cancer Center at Kansas State University
- Midwest of the American Heart Association [0860084Z]
The Gram-positive pathogen Enterococcus faecalis is a leading agent of nosocomial infections, including urinary tract infections, surgical site infections, and bacteremia. Among the infections caused by E. faecalis, endocarditis remains a serious clinical manifestation and unique in that it is commonly acquired in a community setting. Infective endocarditis is a complex disease, with many host and microbial components contributing to the formation of bacterial biofilm-like vegetations on the aortic valve and adjacent areas within the heart. In the current study, we compared the pathogenic potential of the vancomycin-resistant E. faecalis V583 and three isogenic protease mutants (Delta gelE, Delta sprE, and Delta gelE Delta sprE mutants) in a rabbit model of enterococcal endocarditis. The bacterial burdens displayed by GelE(-) mutants (Delta gelE and Delta gelE Delta sprE mutants) in the heart were significantly lower than those of V583 or the SprE(-) mutant. Vegetations on the aortic valve infected with GelE(-) mutants (Delta gelE and Delta gelE Delta sprE mutants) also showed a significant increase in deposition of fibrinous matrix layer and increased chemotaxis of inflammatory cells. In support of a role for proteolytic modulation of the immune response to E. faecalis, we also demonstrate that GelE can cleave the anaphylatoxin complement C5a and that this proteolysis leads to decreased neutrophil migration in vitro. In vivo, a decreased heterophil (neutrophil-like cell) migration was observed at tissue sites infected with GelE-producing strains but not at those infected with SprE-producing strains. Taken together, these observations suggest that of the two enterococcal proteases, gelatinase is the principal mediator of pathogenesis in endocarditis.
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