Determination of 17 β-Estradiol in Rabbit Plasma by Gas Chromatography with Flame Ionization Detection

Yilmaz and Kadioglu: Determination of 17 beta-Estradiol in Rabbit Plasma This article describes gas chromatography-flame ionization detection method for determination of 17 beta-estradiol in rabbit plasma. 17 beta-estradiol and internal standard progesterone were extracted from plasma using liquid-liquid extraction method. Linearity was found between 0.25 and 20 mu g/ml (r(2)=0.994) for plasma samples. Intra-day and inter-day precision, expressed as the relative standard deviation were less than 5.5%, and accuracy (relative error) was less than 3.5%. The mean recovery of 17 beta-estradiol samples was 94.4%. The limits of detection and quantification of method for plasma samples were 0.10 mu g/ml and 0.15 mu g/ml, respectively. Also, clinically used other 10 drugs were investigated to check for potential interferences and the method was successfully applied to the determination of 17 beta-estradiol in New Zealand white rabbits.