期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷 51, 期 4, 页码 329-335出版社
SPRINGER
DOI: 10.1007/s11626-014-9838-y
关键词
Lhx8; PC12; Proliferation; Cell cycle
资金
- National Natural Science Foundation of China [31271138]
- Natural Science Foundation of Jiangsu Province [BK2012659]
- Natural Science Foundation for Young Scientists of Jiangsu Province [BK20140434]
- Natural Science Foundation of Jiangsu Higher Education Institutions [13KJB310011]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
LIM-homeobox genes play a pivotal function in tissue patterning and differentiation, Lhx8 is a member of LIM-homeobox gene family, and it is selectively expressed in embryonic basal forebrain and is a key factor for the determination of cholinergic cells fate. However, besides cholinergic differentiation, little is known about the potential role of Lhx8 in cell biology. In this study, we transfected Lhx8 complementary DNA (cDNA) into PC12 cell line using lentiviral vectors to acquire the cells which stably expressed high level of Lhx8, and we provide the experimental evidence that overexpression of Lhx8 inhibits cell proliferation and induces cell cycle arrest but not apoptosis in vitro. In conclusion, besides cholinergic differentiation, our results suggest that Lhx8 also plays as a suppressor gene of proliferation in cell biology.
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