期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷 50, 期 9, 页码 802-806出版社
SPRINGER
DOI: 10.1007/s11626-014-9787-5
关键词
Oral squamous cell carcinoma; HBp17/FGFBP-1; FGF-2; 1 alpha,25(OH)(2)D-3; Vitamin D receptor
资金
- Japanese Ministry of Education, Culture, Sports, Science and Technology [24390456, 25463109]
- Grants-in-Aid for Scientific Research [24390456, 25463109] Funding Source: KAKEN
Heparin-binding protein 17/fibroblast growth factor binding protein-1 (HBp17/FGFBP-1, GenBank accession no. NP-005121) is prominent for its role as the chaperone for fibroblast growth factor-2 (FGF-2), which plays a crucial role in angiogenesis as well as promoting tumor growth. HBp17/FGFBP-1 has been proposed as a candidate biomarker for a number of cancers since it is frequently found to be elevated in many cancer types including in the tissue and cell lines of oral squamous cell carcinomas (OSCC). Previously, we reported that 1 alpha,25(OH)(2)D-3 suppressed the HBp17/FGFBP-1 expression in OSCC by inhibiting nuclear factor-kappaB (NF-kappa B) expression via vitamin D3 receptor (VDR). In this paper, to further characterize the inhibitory effect of 1 alpha,25(OH)(2)D-3 on HBp17/FGFBP-1, we examined the cellular localization of HBp17/FGFBP-1 protein and FGF-2 protein in the UE OSCC cell line. We found that the treatment of OSCC cells with 40-nM 1 alpha,25(OH)(2)D-3 suppressed HBp17/FGFBP-1 expression both in the nucleus and cytosol and reduced FGF-2 release into the culture medium. The expression of HBp17/FGFBP-1 and FGF-2 was analyzed by immunofluorescence and enzyme-linked immunosorbent assay (ELISA). In summary, the ability of 1 alpha,25(OH)(2)D-3 to suppress the expression of HBp17/FGFBP-1 and FGF-2 strongly suggests a therapeutic potential as a molecular-targeted anticancer drug for FGF-dependent cancers.
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