期刊
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
卷 50, 期 4, 页码 313-320出版社
SPRINGER
DOI: 10.1007/s11626-013-9705-2
关键词
T cell activation; Lectin; Ig-fusion molecule; Costimulatory signal
资金
- Takeda foundation
- Grants-in-Aid for Scientific Research [25640089, 25281017] Funding Source: KAKEN
T cell activation is regulated by two distinct signals, signals one and two. Concanavalin A (ConA) is an antigen-independent mitogen and functions as signal one inducer, leading T cells to polyclonal proliferation. CD28 is known to be one of major costimulatory receptors and to provide signal two in the ConA-induced T cell proliferation. Here, we have studied the implication of other costimulatory pathways in the ConA-mediated T cell proliferation by using soluble recombinant proteins consisting of an extracellular domain of costimulatory receptors and Fc portion of human IgG. We found that T cell proliferation induced by ConA, but not PMA plus ionomycin or anti-CD3 mAb, is significantly inhibited by herpes virus entry mediator (HVEM)-Ig, even in the presence of CD28 signaling. Moreover, the high concentration of HVEM-Ig molecules almost completely suppressed ConA-mediated T cell proliferation. These results suggest that HVEM might play more important roles than CD28 in ConA-mediated T cell proliferation.
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