期刊
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
卷 34, 期 6, 页码 907-911出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/08923973.2012.670643
关键词
Isorhamnetin 3-O-beta-D-glucopyranoside; Salicornia herbacea; adipogenesis; obesity; AMPK
资金
- Technology Development Program for Fisheries, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea [20100293]
- Institute of Planning & Evaluation for Technology in Food, Agriculture, Forestry & Fisheries (iPET), Republic of Korea [IPET110089-3] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Korea Institute of Marine Science & Technology Promotion (KIMST) [20110019] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In the present study, effect of flavonoid glucopyranoside, isorhamnetin 3-O-beta-D-glucopyranoside, from Salicornia herbacea on adipogenic differentiation were evaluated in 3T3-L1 adipocytes. Confluent 3T3-L1 preadipocytes in medium (0.5 mM methylisobutylxanthine, 0.25 mu M dexamethasone, 5 mu g/mL insulin, and 10% fetal bovine serum [FBS]) were differentiated into adipocytes for 6 days with/without isorhamnetin 3-O-beta-D-glucopyranoside. The presence of isorhamnetin 3-O-beta-D-glucopyranoside effectively suppressed adipogenic differentiation by downregulation of peroxisome proliferator-activated receptor-gamma (PPAR gamma), CCAAT/enhancer-binding proteins (C/EBP alpha), sterol regulatory element-binding protein 1 (SREBP1), and the adipocyte-specific proteins. Moreover, the specific mechanism mediating the effects of isorhamnetin 3-O-beta-D-glucopyranoside was confirmed activation of AMP-activated protein kinase (AMPK). These findings suggest that isorhamnetin 3-O-beta-D-glucopyranoside exerts antiadipogenic activity through AMPK activation. This study should find the nutraceutical value of S. herbacea-derived glucopyranoside, isorhamnetin 3-O-beta-D-glucopyranoside, as potent candidate of antiobesity agent via alleviation of lipid accumulation.
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