期刊
IMMUNOLOGY LETTERS
卷 128, 期 2, 页码 137-142出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2009.12.014
关键词
Apoptosis; Anti-apoptosis; DNA vaccine; HSV-1
类别
资金
- Tarbiat Modares University (Tehran, Iran)
Many approaches have so far been tried to enhance the immunogenicity of DNA vaccine. These include the use of various factors that induce apoptosis or anti-apoptosis effects when co-delivered with DNA vaccine. In the present study, the effects of pro-apoptotic Bax encoding plasmid (pBax) and anti-apoptotic Bcl-X-L encoding plasmid (pBcl-xl), intradermally co-injected with glycoprotein B (gB) of Herpes Simplex Virus (HSV)-1 encoding plasmid (pgB) into the C57BL/6 mice were evaluated. Immune responses of the mice to the antigen were assessed by antibody assay, lymphoproliferative responses as well as cytokine and cytotoxic T-lymphocyte (CTL) assay. Analysis of the humoral and cellular responses showed that the mice immunized with pBax and pgB induced higher levels of antibody and Interleukin as well as stronger lymphocyte proliferative responses and cytotoxic activity compared to those mice received pgB alone. pBcl-xl when intradermally co-injected with pgB showed no significant enhancement in immune responses comparing to pgB. (C) 2009 Elsevier B.V. All rights reserved.
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