期刊
IMMUNOLOGY
卷 133, 期 3, 页码 386-396出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2567.2011.03449.x
关键词
Dectin-1; Fasciola hepatica; macrophages; mannose receptor
类别
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 33326]
- Consejo Nacional de Investigaciones Cientificas y Tecnicas de Argentina [PIP 6327]
- Secretaria de Ciencia y Tecnologia (SeCyT), Universidad Nacional de Cordoba [69/08]
- Ministerio de Ciencia y Tecnologia de la Provincia de Cordoba [2008]
P>Fasciola hepatica releases excretory-secretory products (FhESP), and immunomodulatory properties have been described for the carbohydrates present in these parasite products. The interaction of FhESP with the innate immune cells, such as macrophages, is crucial in the early stage of infection. In this work we observed that peritoneal macrophages from naive BALB/c mice stimulated in vitro with FhESP presented: an increased arginase activity as well as Arginase I expression, and high levels of transforming growth factor-beta and interleukin-10. A similar macrophage population was also observed in the peritoneum of infected mice. A partial inhibition of the immunomodulatory effects described above was observed when macrophages were pre-incubated with Mannan, anti-mannose receptor, Laminarin or anti-Dectin-1, and then stimulated with FhESP. In addition, we observed a partial inhibition of these effects in macrophages obtained from mice that were intraperitoneally injected with Mannan or Laminarin before being infected. Taken together, these results suggest the participation of at least two C-type lectin receptors, mannose receptor and Dectin-1, in the interaction of FhESP with macrophages, which allows this parasite to induce immunoregulatory effects on these important innate immune cells and may constitute a crucial event for extending its survival in the host.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据