期刊
IMMUNOLOGY
卷 130, 期 2, 页码 181-192出版社
WILEY
DOI: 10.1111/j.1365-2567.2009.03222.x
关键词
asthma; CD4+CD25+regulatory T cells; lung; skin; vitamin D
类别
资金
- National Health and Medical Research Council of Australia [458612]
- Cancer Council of Western Australia
- University of Western Australia
P>The severity of allergic diseases may be modified by vitamin D. However, the immune pathways modulated by the active form of vitamin D, 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3], are yet to be fully elucidated. In this study, naturally occurring CD4+ CD25+ cells from the skin-draining lymph nodes (SDLN) of mice treated with topical 1,25(OH)(2)D-3 had an increased ability to suppress T helper type 2 (Th2) -skewed immune responses. CD4+ CD25+ cells transferred from mice treated with topical 1,25(OH)(2)D-3 into ovalbumin (OVA) -sensitized mice challenged intranasally with OVA 18 hr later, significantly suppressed the capacity of airway-draining lymph node (ADLN) cells to proliferate and secrete cytokines in response to further OVA stimulation ex vivo. The CD4+ CD25+ cells from 1,25(OH)(2)D-3-treated mice also reduced airway hyperresponsiveness and the proportions of neutrophils and eosinophils in bronchoalveolar lavage fluid (BALF). To test the effect of 1,25(OH)(2)D-3 on cells able to respond to a specific antigen, CD4+ CD25+ cells were purified from the SDLN of OVA-T-cell receptor (TCR) transgenic mice treated 4 days earlier with topical 1,25(OH)(2)D-3. CD4+ CD25+ cells from OVA-TCR mice treated with 1,25(OH)(2)D-3 were able to alter BALF cell content and suppress ADLN responses to a similar degree to those cells from non-transgenic mice, suggesting that the effect of 1,25(OH)(2)D-3 was not related to TCR signalling. In summary, topical 1,25(OH)(2)D-3 increased the regulatory capacity of CD4+ CD25+ cells from the SDLN to suppress Th2-mediated allergic airway disease. This work highlights how local 1,25(OH)(2)D-3 production by lung epithelial cells may modulate the suppressive activity of local regulatory T cells.
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