期刊
IMMUNOGENETICS
卷 61, 期 11-12, 页码 703-716出版社
SPRINGER
DOI: 10.1007/s00251-009-0399-2
关键词
MHC class I; Tapasin; Antigen presentation/processing; TAP transporter; HLA-B*3501; HLA-B*3503; HLA-B*4402; HLA-B*4405; HLA-B*5701
资金
- NIH [AI44155]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI044115] Funding Source: NIH RePORTER
Residue 116 of major histocompatibility complex (MHC) class I heavy chains is an important determinant of assembly, that can influence rates of ER-Golgi trafficking, binding to the transporter associated with antigen processing (TAP), tapasin dependence of assembly, and the efficiency and specificity of peptide binding. Here, we investigated assembly and peptide-binding differences between HLA-B*3501(S116) and HLA-B*3503(F116), two alleles differing only at position 116 of the MHC class I heavy chain, that are associated respectively with normal or rapid AIDS progression. A reduced intracellular maturation rate was observed for HLA-B*3503 in HIV-infected and uninfected cells, which correlated with enhanced binding of HLA-B*3503 to TAP. No significant differences in the intrinsic efficiency of in vitro peptide binding by HLA-B*3501 and HLA-B*3503 were measurable with several common peptides or peptide libraries, and both allotypes were relatively tapasin-independent for their assembly. However, thermostability differences between the two allotypes were measurable in a CD4(+) T cell line. These findings suggest that compared to HLA-B*3501, a reduced intracellular peptide repertoire for HLA-B*3503 could contribute to its slower intracellular trafficking and stronger association with rapid AIDS progression.
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