期刊
IMMUNOBIOLOGY
卷 218, 期 2, 页码 225-231出版社
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.imbio.2012.04.012
关键词
Airway epithelium; Bronchoalveolar lavage; Chronic bronchitis; Ex-smokers; Regulatory T cell
类别
资金
- Swedish Heart-Lung Foundation
- King Oscar II Jubilee Foundation
- Mats Kleeberg Foundation
- King Gustaf V's and Queen Victoria's Freemasons' Foundation
- Hesselmans Foundation
- Karolinska Institutet
- Swedish Research Council
- Stockholm County Council
T cells are accumulated in the lungs of chronic obstructive pulmonary disease (COPD) patients. Intraepithelial T cells, expressing the integrin alpha E (CD103) beta 7, and regulatory T cells have been implicated in pathogenesis of the disease. We asked whether COPD patients and smokers have altered frequencies of these T cells and if their phenotypes differ. A total of 40 never-smokers, 40 smokers with normal lung function and 38 COPD patients (GOLD land II), of which 11 were ex-smokers, were included. T cells in bronchoalveolar lavage (BAL) fluid and peripheral blood were analysed for the expression of CD103, FOXP3 and markers of activation and differentiation using multi-colour flow cytometry. Smokers, regardless of airway obstruction, had significantly more CD8+CD103+ cells in their BAL fluid compared to never-smokers but less of those cells were CD27+CD69-. Smokers, in particular those with chronic bronchitis, had a higher percentage of CD4+FOXP3+ T-regulatory BAL cells compared to never-smokers and COPD ex-smokers. Chronic cigarette smoking leads to an accumulation of CD8+ T cells with an altered phenotype in the airway epithelium. The increased frequency of regulatory T cells may influence the ability to regulate smoke-induced inflammation which could be decisive for disease development. Our results further indicate a reversibility of smoke-induced changes. (C) 2012 Elsevier GmbH. All rights reserved.
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