期刊
IMMUNITY
卷 38, 期 2, 页码 237-249出版社
CELL PRESS
DOI: 10.1016/j.immuni.2012.09.012
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类别
资金
- NIH [AI073457, CA150975, AI0178897, AI097052]
- Dana Foundation
- MGH Fund for Medical Discovery Fellowship
- MGH ECOR Tosteson Postdoctoral Fellowship Award
Interactions with antigen-presenting cells (APCs) interrupt T cell migration through tissues and trigger signaling pathways that converge on the activation of transcriptional regulators, including nuclear factor of activated T cells (NFAT), which control T cell function and differentiation. Both stable and unstable modes of cognate T cell-APC interactions have been observed in vivo, but the functional significance of unstable, serial contacts has remained unclear. Here we used multiphoton intravital microscopy in lymph nodes and tumors to show that while NFAT nuclear import was fast (t(1/2) (max)similar to 1 min), nuclear export was slow (t(1/2)similar to 20 min) in T cells. During delayed export, nuclear NFAT constituted a short-term imprint of transient TCR signals and remained transcriptionally active for the T cell tolerance gene Egr2, but not for the effector gene Ifng, which required continuous TCR triggering for expression. This provides a potential mechanistic basis for the observation that a predominance of unstable APC interactions correlates with the induction of T cell tolerance.
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