期刊
IMMUNITY
卷 32, 期 5, 页码 714-725出版社
CELL PRESS
DOI: 10.1016/j.immuni.2010.04.013
关键词
-
类别
资金
- Max-Planck-Society
- German Research Foundation
- Agency for Science, Technology and Research (A*STAR), Singapore
The transcription factor early B cell factor-1 (Ebf1) is a key determinant of B lineage specification and differentiation. To gain insight into the molecular basis of Ebf1 function in early-stage B cells, we combined a genome-wide ChIP sequencing analysis with gain- and loss-of-function transcriptome analyses. Among 565 genes that are occupied and transcriptionally regulated by Ebf1, we identified large sets involved in (pre)-B cell receptor and Akt signaling, cell adhesion, and migration. Interestingly, a third of previously described Pax5 targets was found to be occupied by Ebf1. In addition to Ebf1-activated and -repressed genes, we identified targets at which Ebf1 induces chromatin changes that poise the genes for expression at subsequent stages of differentiation. Poised chromatin states on specific targets could also be established by Ebf1 expression in T cells but not in NI H 3T3 cells, suggesting that Ebf1 acts as a pioneer factor in a hematopoietic chromatin context.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据