期刊
IET NANOBIOTECHNOLOGY
卷 12, 期 8, 页码 1118-1124出版社
WILEY
DOI: 10.1049/iet-nbt.2018.5073
关键词
transmission electron microscopy; wounds; nanoparticles; ultraviolet spectra; reduction (chemical); particle size; nanofabrication; gold; X-ray diffraction; antibacterial activity; microorganisms; visible spectra; nanomedicine; biomedical materials; Fourier transform infrared spectra; biological activity; gold nanoparticles; antibacterial agent; therapeutic approach; colour changes; ultraviolet-visible spectroscopy; transmission electron microscopy; gold reduction; antimicrobial properties; Fourier transform infrared spectroscopy analyses; disc-diffusion assay; green synthesis; Tragopogon dubius leaf; in vitro antibacterial activity; in vivo antibacterial activity; X-ray diffraction; particle sizes; functional groups; standard microdilution assay; burn wounds; S; aureus; tetracycline; bacterial reduction; wound healing; wavelength 560; 0 nm
资金
- Shiraz University
- Natural Antimicrobial Centre of Excellence (NACE)
Currently, the use of green' synthesised nanoparticles with environmentally friendly properties is considered a novel therapeutic approach in medicine. Here, the authors evaluated gold nanoparticles (AuNPs) conjugated with Tragopogon dubius leaf extract and their antibacterial activity in vitro and in vivo. Colour changes from yellow to dark brown and a peak at 560nm on ultraviolet-visible spectroscopy confirmed the formation of nanoparticles. Additionally, transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy analyses were performed to determine particle sizes and functional groups involved in gold reduction. Moreover, using standard micro-dilution and disc-diffusion assays against Klebsiella pneumoniae, Bacillus cereus, Escherichia coli, and Staphylococcus aureus, the antimicrobial properties of synthesised AuNPs were investigated. To confirm antibacterial activity, synthesised AuNPs were applied in a rat model on burn wounds infected with S. aureus, and the nanoparticles were as effective as tetracycline in bacterial reduction and wound healing. In conclusion, the synthesis of AuNPs with aqueous T. dubius extract was rapid, simple, and inexpensive, and the synthesised nanoparticles had significant antibacterial activity in vitro and in vivo.
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