期刊
IET NANOBIOTECHNOLOGY
卷 8, 期 2, 页码 102-110出版社
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2012.0023
关键词
biological techniques; lab-on-a-chip; magnetic particles; magnetic separation; magnetic switching; microchannel flow; moulding; nanobiotechnology; nanoparticles; permanent magnets; magnetically actuated separation; tangential microfluidic channels; magnetic nanoparticles; MNP; neodymium permanent magnet; polydimethylsiloxane tangential microchannels; micromoulding; flow rate; nonmagnetic polystyrene particles; biomolecules; microfluidic lab-on-a-chip systems
资金
- National Science Foundation [CMMI-1030289]
- National Natural Science Foundation of China [31101274]
A novel continuous switching/separation scheme of magnetic nanoparticles (MNPs) in a sub-microlitre fluid volume surrounded by neodymium permanent magnet is studied in this work using tangential microfluidic channels. Polydimethylsiloxane tangential microchannels are fabricated using a novel micromoulding technique that can be done without a clean room and at much lower cost and time. Negligible switching of MNPs is seen in the absence of magnetic field, whereas 90% of switching is observed in the presence of magnetic field. The flow rate of MNPs solution had dramatic impact on separation performance. An optimum value of the flow rate is found that resulted in providing effective MNP separation at much faster rate. Separation performance is also investigated for a mixture containing non-magnetic polystyrene particles and MNPs. It is found that MNPs preferentially moved from lower microchannel to upper microchannel resulting in efficient separation. The proof-of-concept experiments performed in this work demonstrates that microfluidic bioseparation can be efficiently achieved using functionalised MNPs, together with tangential microchannels, appropriate magnetic field strength and optimum flow rates. This work verifies that a simple low-cost magnetic switching scheme can be potentially of great utility for the separation and detection of biomolecules in microfluidic lab-on-a-chip systems.
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