期刊
IET NANOBIOTECHNOLOGY
卷 7, 期 4, 页码 135-139出版社
INST ENGINEERING TECHNOLOGY-IET
DOI: 10.1049/iet-nbt.2012.0032
关键词
antibacterial activity; cellular biophysics; decomposition; fluorescence; II-VI semiconductors; microorganisms; nanofabrication; nanoparticles; pH; photoluminescence; wide band gap semiconductors; X-ray diffraction; zinc compounds; nanomedicine; zinc sulphide; nanocomplex; magnetite zinc oxide; Brevundimonas diminuta; Pseudomonas stutzeri; chemical reactions; decomposition; zinc acetate; hot-injection; heating-up method; semiconductor nanoparticles; sphalerite; high-zinc tolerant bacteria; nanozinc production; microorganisms; gram negative bacterium; nanomagnetite zinc oxide; bacterial surface; zinc metal; yellow-white biofilm; X-ray diffractometer; pH; antibacterial activity; photoluminescence microscopy; fluorescent microscopy; size 22 nm; size 48; 29 nm; temperature 293 K to 298 K; temperature 23 degC; ZnO; ZnS
ZnO (Zincite) nanoparticle has many industrial applications and is mostly produced by chemical reactions, usually prepared by decomposition of zinc acetate or hot-injection and heating-up method. Synthesis of semi-conductor nanoparticles such as ZnS (Sphalerite) by ultrasonic was previously reported. In this work, high-zinc tolerant bacteria were isolated and used for nano zinc production. Among all isolated microorganisms, a gram negative bacterium which was identified as Brevundimonas diminuta could construct nano magnetite zinc oxide on bacterial surface with 22 nm in size and nano zinc with 48.29 nm in size. A piece of zinc metal was immersed in medium containing of pure culture of B. diminuta. Subsequently, a yellow-white biofilm was formed which was collected from the surface of zinc. It was dried at room temperature. The isolated biofilm was analysed by X-ray diffractometer. Interestingly, the yield of these particles was higher in the light, with pH 7 at 23 degrees C. To the best of the authors knowledge, this is the first report about the production of nano zinc metal and nano zinc oxide that are stable and have anti-bacterial activities with magnetite property. Also ZnS (sized 12 nm) produced by Pseudomonas stutzeri, was studied by photoluminescence and fluorescent microscope.
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