期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 49, 期 -, 页码 485-492出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2015.01.011
关键词
Amylose; Poly(L-lysine) dendron; Click conjugation; Gene delivery; Cytotoxicity
资金
- National Natural Science Foundation of China [51273216, 21074152, J1103305]
- Doctoral Research Program of Education Ministry in China [20090171110023, 20130171120095]
- Key Project of Scientific and Technical Innovation for Universities in Guangdong Province [cxzd1102]
- Natural Science Foundation of Guangdong Province [S2013010012549, S2013040014401]
Although amylose as a naturally-occurring helical polysaccharide has been widely used for biomedical applications, few studies have dealt with its chemical modification for non-viral gene delivery. In this work, the click modification of amylose by poly(L-lysine) dendrons was carried out and then characterized by Fourier transform infrared spectroscopy, wide-angle X-ray diffraction and elemental analyses. Such a modified polysaccharide exhibited excellent ability to condense plasmid pMSCV-GFP-PARK2 into compact and spherical nanoparticles. Moreover, it displayed much lower cytotoxicity when compared to branched polyethylenimine (bPEI, 25 kDa), a commercially available gene vector. Similar to bPEI, it had a dose-dependent gene transfection activity in human embryonic kidney 293T cells, as observed by confocal laser scanning microscopy and flow cytometry. At each optimized N/P ratio, the percentage of transfected cells by this modified polysaccharide was found to be comparable to that by bPEL. Western blot and cell apoptosis analyses confirmed its effectiveness for the delivery of plasmid pMSCV-GFP-PARK2 to 293T cells. (C) 2015 Elsevier B.V. All rights reserved.
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