期刊
IEEE TRANSACTIONS ON NANOBIOSCIENCE
卷 11, 期 1, 页码 15-21出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TNB.2011.2159621
关键词
Conducting fibers; electrical stimulation; neural tissue engineering; nerve growth factor
资金
- NIH [R01EB004429]
- Institute for Critical Technologies and Sciences at Virginia Tech.
Engineered scaffolds simultaneously exhibiting multiple cues are highly desirable for neural tissue regeneration. To this end, we developed a neural tissue engineering scaffold that displays submicrometer-scale features, electrical conductivity, and neurotrophic activity. Specifically, electrospun poly(lactic acid-co-glycolic acid) (PLGA) nanofibers were layered with a nanometer thick coating of electrically conducting polypyrrole (PPy) presenting carboxylic groups. Then, nerve growth factor (NGF) was chemically immobilized onto the surface of the fibers. These NGF-immobilized PPy-coated PLGA (NGF-PPyPLGA) fibers supported PC12 neurite formation (28.0 +/- 3.0% of the cells) and neurite outgrowth (14.2 mu m median length), which were comparable to that observed with NGF (50 ng/mL) in culture medium (29.0 +/- 1.3%, 14.4 mu m). Electrical stimulation of PC12 cells on NGF-immobilized PPyPLGA fiber scaffolds was found to further improve neurite development and neurite length by 18% and 17%, respectively, compared to unstimulated cells on the NGF-immobilized fibers. Hence, submicrometer-scale fibrous scaffolds that incorporate neurotrophic and electroconducting activities may serve as promising neural tissue engineering scaffolds such as nerve guidance conduits.
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